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By Hiroaki Sakurai

Using batch reactors equipped with CO and CO2 gas sensor, CO oxidation over Au catalysts in room air has been investigated and compared with Pt catalysts. For both Au and Pt catalysts, the logarithm plot of CO concentration data during initial 10 min are well fitted by straight line, which mean the first order dependence on the CO concentration between 10 and 10,000ppm. The rate constant value (k) over Au and Pt catalyst generally decrease with increasing initial CO concentration above 100ppm. The value decrease especially rapidly between 100ppm and 1,000ppm over Pt catalysts, which should be due to the strong adsorption of CO on Pt. The k value over Au catalyst has become ten times or more as large as the Pt catalysts at 1,000ppm. Already for the initial concentration of 100ppm CO, the strongly adsorbed CO on Pt metal has been clearly observed by FT-IR during the reaction over Pt/Al2O3. A part of the strongly adsorbed CO has been remained on Pt after the disappearance of gas phase CO, and has gradually changed to gas phase CO2. Such strongly adsorbed species on Au has never been observed on Au/TiO2 under the same reaction conditions. These features of Au catalysts are favorable for indoor air purification.

Oct 1, 2003

By H Li

"An EXTENDED ABSTRACT is available for download. A full-length paper was not prepared for this presentation. The central Nanling region in South China experienced multistage granite intrusive events and corresponding metallogenic processes during the Early Yanshanian period (190–140 Ma). In this study, a wide variety of published data on age determination, petrological characteristics, major and trace elements compositions, whole rock Sr–Nd contents and zircon Hf isotopic composition of the representative granite composites were systematically collected to estimate their cooling-uplift rates, granite evolution processes and mineral deposit formation. These granites have been classified into two groups based on their age and geochemical variations: Group 1 (180–165 Ma) and Group 2 (165–150 Ma). Major and trace element geochemistry suggests that Group 2 experienced more intense magmatic evolution and differentiation than Group 1. In addition, Group 1 granitoids are classified as A1-type alkaline rocks with faster cooling and uplift rates, whereas Group 2 granites are considered as A2-type aluminous rocks with slower cooling and ascent rates. Furthermore, the Group 1 granitoids have younger Nd and Hf two-stage model ages but higher eNd(t) and eHf(t) values, indicating influence from crust–mantle interaction. In contrast, the Group 2 granites show different values, suggesting stronger upper crustal contamination or assimilation. The slowly cooled and ascended Group 2 granites experienced much heavier crustal contamination (or assimilation) and crystal fractionation, contributing to the large W–Sn mineralisation in the central Nanling region. The Early Mesoproterozoic (1.6–1.4 Ga) crustal basement of the Cathaysia Block in South China is the ore source bed for the world-famous Nanling W–Sn polymetallic mineralisation.CITATION:Li, H, 2015. Where is the source of the world-famous Nanling W–Sn polymetallic mineralisation?, in Proceedings PACRIM 2015 Congress, pp 619–622 (The Australasian Institute of Mining and Metallurgy: Melbourne)."

Mar 18, 2015

By D. Diering

Having agreed to perform this task, I naturally started to wonder what on earth I was going to talk about, and on reviewing the literature I was surprised at how little I knew and how much I?d forgotten about the origins of this industry. For example: ?I?d forgotten that the Chinese invented black powder at least 1 000 years ago, back in the 10th century (although some say this invention occurred 2 000years ago). ?I didn?t know that Roger Bacon was the first person in the Western World to give exact directions for the making of gunpowder in 1242 and to understand its potential. Pepper?s Playbook of Metals quotes:?...above all, he is named as the discoverer of gun-powder, a material which has brought more wonderful changes in the great family of man than the most potent elixir that could have been devised to prolong life?.

Jan 1, 2000

By R. K. Rath

Adsorption, electrokinetic and flotation studies were carried out. The maximum density of adsorption of guar gum was obtained at pH 7.5 on sphalerite and at pH 11.5 on galena. Flotation recovery of sphalerite was reduced only marginally by the presence of guar gum in the pH range investigated, whereas recovery of galena was reduced to about 20% at pH 12. Differential flotation tests on binary mixtures indicated that 10 ppm of guar gum can depress galena and separate it from sphalerite at around pH 12.1. Co-precipitation tests confirmed the formation of a metal ion-guar gum complex in the bulk solution, suggesting that this is the precursor to adsorption. The adsorption isotherms for both minerals followed Langmuirian behaviour, but adsorption of guar gum on galena was significantly higher than on sphalerite and dissolution tests indicated that the concentration of Pb ions released from galena was an order of magnitude greater than that of Zn ions dissolved from sphalerite

Jun 21, 1905

By G R. Howard, G Back

Management of information systems for data collection is essential to maintain data integrity and continuity, and manage loss control on a large world-class mine such as Telfer Gold Mine. This paper aims to describe the individual systems and protocols of geological data capture at Telfer, then focus on Quality Assurance Quality Control data as an example of loss control in practice. Loss control systems are required to provide capacity, flexibility, structure and management tools to enhance data interrogation. The data that have been collected can then provide geological, financial, environmental, and contractual information to optimise management of business best practice. Telfer Gold Mine is a wholly owned and operated subsidiary of Newcrest Mining Limited. The mine is located in a remote area of NW Western Australia, and has been operating for 20 years. Geological data have been collected at Telfer since 1973. The data include drilling, assay, geological, survey, structural and geophysical data. Data management has evolved through many systems with data stored on many different hardware and software platforms. Commencing in 1995, collation, standardisation and validation of the historic data was completed. This has resulted in the first Access based drill hole database incorporating all available drilling data. This drill hole database, now hosted in SQL Server, is the hub for all geology related data management systems. The Telfer Gold Mine Geology Department has developed a series of inter-related database systems in order to manage the core geology business issues. Databases relating to drill hole planning, drill contract management, geological logging, accounting (standard costing, forecasting, invoice reconciliation), quality control (QAQC), and environment rehabilitation have all been developed to manage loss control. Management effort to provide adequate data systems and protocols has driven the system from concept to utilisation. The improved productivity, data integrity and data analysis capabilities provided by the new database systems at Telfer have allowed for greater confidence in decision making. Systems such as the drill hole and QAQC databases, when utilised in the proper manner provide up-to-date data analysis for resource audits and contract negotiations. The loss control systems are essential to maintain confidence in data integrity and hence directly effect the future of mining at Telfer.

Jan 1, 2000

By L Forrester

The tube bundle system is an integral part of an underground coalmine’s gas monitoring system. The maintenance of these systems is vital to ensure the quality of the data produced. Integrity testing of the tube bundle lines is required monthly, as specified in Standards Australia AS/NZ 2290.3 (1990). The various forms of integrity testing are reviewed, including pressure testing and introduction of a known gas. This paper provides a recommended and standardised methodology for those techniques where none currently exists. A strengths, weaknesses, opportunities, threats (SWOT) analysis is performed. The parameters considered for the SWOT analysis include resourcing required by the mines, time for the testing to be performed, and quality of the results produced. The suitability of such integrity testing protocols to be used in a mine emergency situation is also evaluated.CITATION:Forrester, L, 2017. Tube bundle integrity testing methodologies, in Proceedings Australian Mine Vent Conference 2017, pp 129–136 (The Australasian Institute of Mining and Metallurgy: Melbourne).

Aug 28, 2017

Operational effectiveness is fundamental to any operation, becoming highly topical in those experiencing less than optimal output from their fleets. It is all encompassing aiming to identify those factors that could be improved within production and suggesting solutions in order to optimise the existing fleet and obtain the greatest capital return on expenditure.Argyle Diamond Mine (ADM) is a large open cut diamond mine located in the East Kimberley region of Western Australia, approximately 180 km south of Kununurra. It is owned and operated by Rio Tinto on a two week on/two week off roster fly-in/fly-out from Perth or drive in/drive out from Kununurra. Through benchmarking the operation between January and June 2002, a study was undertaken allowing identification of areas falling below target. Following this, analysis of annualised hours and production downtimes were undertaken to quantify the potential gains of improvements in these areas. Strategies were then identified as stated in the 2002 mine plan, in operational effectiveness meetings, and by staff members. This project aimed to maximise direct working hours through determination of the main issues hindering performance. External and internal factors were considered and a continuous improvement program (CIP) was developed. The CIP aimed at internal site-based improvement teams to identify and implement solutions for improvement on a day-to-day basis.

Jan 1, 2003

By S. Banisi, M. Pourkani, A. Kargar, D. Hamidi, M. Sarvi

"Flotation circuit of the Sarcheshmeh copper mine, which processes 40,000 t/d of ore at 0.9% Cu, consists of rougher, cleaning and recleaning stages. To study the stage recovery of copper, one of the eight identical flotation banks was selected for sampling. It was observed that 17% of copper goes to the tailings and the lowest copper recovery was for particles coarser than 105 microns. The third five-cell unit of this bank not only did not recover appreciable amount of copper but also decreased the concentrate grade. The grade of the last five cells was lower than the feed grade suggesting that mixing of the concentrates of the three units was not beneficial. To alleviate the problem, the concentrate of the first four-cell unit was directed to the cleaners feed box (i.e., by- passing the regrind mill). This increased the overall copper recovery by 1.7 %. Also rather than the current practice of adding the reagents only at the feed tank (i.e., 100-0-0%), various combinations of stage addition were examined. It was found that 75-25-0% addition regime could increase the total copper recovery by 1.3%. At the recleaning stage in order to increase the final copper concentrate grade, a froth washing system was used. To ease the maintenance of the system, a shower type washing system was installed. The optimum wash water superficial flowrate was found to be 0.06 cm/s. Continuos sampling of the recleaners with and without wash water over a period of two months showed an increase of 1.5% in the final copper concentrate grade at constant overall copper recovery.INTRODUCTIONThe Sarcheshmeh copper ore body that may rank as third or fourth largest in the world contains 1 billion tonnes averaging 0.9% copper and 0.035% molybdenum. It is located in southeastern Iran and has been processing 40 000 t/d of ore since 1982. The mine produces 100 000 tonnes of copper and 2200 tonnes of molybednite concentrate per year.After three stages of crushing, the ore with a F80 (80% passing size) of 12.7 mm is fed to eight parallel ball mills in closed circuit with cyclones to produce a product 70% finer than 75 microns. The concentrate of rougher flotation circuit is ground; rougher tails constitute the main component of the plant final tailings. A concentrate with an average grade of 31% Cu (chalcocite and chalcopyrite) is obtained after cleaning and re-cleaning stages. Total recovery of the plant varies between 83% and 87%, depending on operating conditions and ore type."

Jan 1, 2001

By J. Thompson

Paper written on project work carried out in partial fulfilment of B. Eng (Mining Engineering) degree A study into the stability of strike gullies at TauTona mine was to be carried out. In reaching the conclusions to follow, a three-stage process was followed. ?A literature study as well as discussions with management and workers alike to gain their inputs and perceptions. ?An ESH Department survey into the history of transgression from proper mine standard in TauTona strike gullies. Unfortunately records were found to be scarce and obscure and the importance of better inter-departmental recordings cannot be stressed enough. ?An underground survey of 11 pre-selected gullies distributed in both the UCL and LCL reef horizons. This stage is to carry the greatest weight in the points to be brought forward in this report. The process highlighted and ranked the following areas as the most critical role players in the deterioration of strike gullies: ?Poor blasting practice ?Sub-standard siding depth and lead-lags ?Split sets ?Off line gully development ?Adverse geological features. The following has been suggested to the mine to help reduce strike gully instability. ?Where possible, maintain lead-lags below 7 m?else increase the siding depth to suit. ?Decrease the gully width standard to 1.2 m (especially in ?high risk areas?). ?Discourage drillers from drilling side holes in gully face blast pattern. This will allow for time in which tendon support can be kept up to date. ?Ensure split sets are installed 1 m behind the gully face so as to prevent opening up of fractures. ?Utilize the centre split set in the 1:2:1 pattern to maintain the proper gully line. ?When adverse conditions prevail, it is necessary to apply a special support plan not only to the panel face, but also (just as importantly) to the gully as well. Shotcreting of the gully hanging is a suitable long-term consideration for providing the additional a real coverage needed. List of Abbreviations [CL Carbon Leader M/O Mine Overseer UCL Upper Carbon Leader FOG Fall of Ground LCL Lower Carbon Leader H/W Hanging Wall ERR Energy Release Rate F/W Foot Wall WDL Western Deep Levels ESH Environmental Safety and Health ]

Jan 1, 2002

By F. D. Ford, Z. Dai, A. Byers

Manitoba Mining and Milling operations consist of three underground mines (Thompson T1, T3 and Birchtree), feeding the Thompson Mill. Three years ago, a collaborative effort involving Mines Exploration, Mines Project Development Group, Mill Technical Services and Vale Base Metals Technical Excellence Center was initiated to establish and verify the cause of high variability in concentrate quality and Ni recovery at the Thompson Mill. One outcome of this work was a program to systematically characterize the mineralogy and metallurgical performance of current and future orebodies feeding the mill. Mineralogy results show that high value enabling ores typically have a high pentlandite to pyrrhotite ratio and benign silicate mineralogy (mostly quartz and feldspar from sedimentary host rocks), while metallurgically challenging ores contain some combination of pyrite and/or hexagonal (non-magnetic) pyrrhotite and/or elevated quantities of talc (with associated carbonates) derived from altered ultramafic host rocks. The metallurgical performance of these two ore types has been simulated by an Excel particle flotation model, which calculates the relative floatability of particles based on the particle’s mineral composition and mineral locking determined from MLA images. The relationship between these mineral properties measured by MLA and metallurgical performance was established using 13 monthly composite samples from Thompson Mill. The model was then validated with results of laboratory flotation experiments on individual orebodies submitted for evaluation. The validated model can be used to predict plant metallurgical performance based on individual ore bodies feeding the mill. Individual orebody’s grade-recovery curves are assumed to be additive, and can be weighted in proportion to the mill feed to predict mill recovery over the same time period. The ore mixing can be done with a simple Excel spreadsheet and comes with a graphical output for easy visualization of the resulting grade-recovery curve.

Jan 1, 2015

By Holmes, George H. Jr.

The Cherry Creek tungsten district was explored by the Bureau of Mines from April 16 to August 13, 1942. This district is in north-central White Pine County, Nev., 50 miles north of Ely. Principal work was done on the property of the Cherry Creek Tungsten Mining Co. and at the adjacent Chance mine, although the entire district was examined and mapped. The exploratory work was done in cooperation with the Federal Geological Survey and comprised surveying, trenching, sampling, long-hole and core drilling, rehabilitation of mine workings, road building, drifting, and shaft sinking. The more important tungsten occurrences were explored by trenching and diamond drilling. Sixteen holes, having a total length of 2,515 feet, were drilled. The project resulted in partial development of three new tungsten deposits and small increases in the reserve tonnage of two scheelite-bearing lenses that were being mined by the company. The following pages summarize the history of the district, outline the ownership of the properties explored, and describe the physical features and mineral deposits. Mining and milling practices at the Cherry Creek tungsten mine are outlined, and the plan and results of project work are presented in detail together with surface maps, assay maps, and drill-hole logs.

Jan 1, 1950

By Edward (Ted) Dowey

City Water Tunnel No. 3 is being built in order to provide a redundant system for delivering 4,542,500 m3 (1.2 billion gallons) of water per day throughout the five boroughs of New York City. The present tunnels, City Tunnel No. 1 and 2 (Figure 1), were put in service in 1917 and 1936 respectively, and both have been in continuous service. Tunnels No. 1 and 2 can-not be shut down for inspection or repair until City Tunnel No. 3 has been completed. City Tunnel No. 3 has two stages (Figure 1).Stage One is 20.1 km (12.5 mi) long and is presently delivering drinking water to the boroughs of the Bronx and Manhattan. Stage Two consists of the Brooklyn-Queens Tunnel 17.7 km (11 mi) and the Manhattan Tunnel 14 km (9 mi). The Brooklyn-Queens leg is complete and is set to be put in service soon. The Manhattan Tunnel is being constructed and is the subject of this paper. The Manhattan Tunnel (Figure 1) is comprised of three legs: the north, south and east legs. The con-tract includes the excavation of 8,976 m (29,500 ft) of tunnel and the lining of 14,561 m (47,773 ft) of tunnel. The south tunnel, 5,705 m (18,720 ft), was excavated but not lined, under a previous contract. The tunnel ranges from 181 m (600 ft) to 152 m (500 ft) in depth in the bedrock under Manhattan. The tunnel contract also includes the construction of nine new shafts, 161 m (530 ft) average depth, and the completion of a tenth shaft (the previously built construction shaft). The Manhattan Tunnel contract was awarded to the sole bidder Schiavone/Shea/Frontier Kemper Joint Venture (SSF) for $668,532,680.00, on January 31, 2005. The Manhattan Tunnel was designed ?in house? by the New York City Department of Environmental Protection (DEP) and the construction management was provided by a combined staff of the DEP and a joint venture of Jenny Engineering and URS.

Jan 1, 2008

By A. Rosenfeld

The feasibility of applying laser induced optical emission spectroscopy to the high speed sorting of mixed alloy aluminum scrap is investigated. The basic spectroscopy for analysing aluminum alloys for the major alloying elements over their full concentration ranges is established. Materials handling issues are also described and the components of an overall sorting system are presented.

Jan 1, 1995

By G. H. Babcock, D. A. Knight, V. G. Medina

"Production commenced at the Golden Sunlight Mine in 1983 and at Kidston Gold Mine in 1985. These projects were both constructed under the management of the Placer Dome Inc. (Technical Services) group of Vancouver, B.C.Golden Sunlight uses a conventional rod mill/ball mill grinding circuit while Kidston uses a SAG /ball mill, and later on, SABC circuit. Since the hardness of the ore and the fineness of the primary grind in both mines are about the same, a meaningful cost comparison can be made. The comparison is made on the basis of capital and operating costs.The result of this study indicates that a significant capital cost savings can be achieved in favour of the SAG /ball mill circuit when the ore is amenable to semi-autogenous grinding. With a good maintenance program, the operating cost for the SAG /ball mill circuit could also be lower that the conventional rod mill/ball mill circuit.HISTORYGolden Sunlight MineGolden Sunlight is a 5 530 t/d gold mine located near Whitehall, a small town 60 km east of Butte, Montana. Originally optioned by PDI U.S. in 1958, the development of the property waited until 1981 when economic conditions became favorable for the project to proceed.Semiautogenous grinding tests were conducted during the final stages of the feasibility study. The hard near surface ore was not quite amenable to semi-autogenous grinding. The best result produced from a series of tests using a 1.83 m diameter mill was a semiautogenous work index of 39. This is substantially higher than the 22 Bond work index of the sample used in the testwork. A conventional SAG/ball mill circuit for this ore will be extremely inefficient, and for this reason, this circuit was rejected in favour of a conventional rod mill/ball mill circuit."

Jan 1, 1991

By Ivan Weber

Asking the question, “What do the world’s leading corporations and cutting-edge practitioners of sustainability put forth to guide a given mine site for planning and action toward ‘sustainability’?”, this paper explores the spectrum of methodologies and mindsets in available literature, then constructs an example for the large near-closure facility at Kennecott Utah Copper. Values, opportunities and constraints spring from physical, unutilized byproducts, from critical ecosystems and wildlife populations, from location relative to those ecosystems and proximate communities, and from the political will to perform adequate feasibility investigations. Patience, determination and thoroughness are imperative. Identifying most productive and, simultaneously, most protective and restorative, practical methods to approach ‘sustainability’ has become a central mission of industry and business, worldwide. A survey of recent international initiatives finds no single approach that establishes either a readily adaptable methodology or an appropriate set of ‘attention’ points, or agenda, to guide individual mining operations at specific locations toward the elusive but essential goal of ‘sustainable’ mining. Untying this convoluted knot of contending factors requires willingness to recognize and work within ecological constraints for community long-term needs. Occasionally, the task requires the sword of Alexander the Great. Through integration that is unflinchingly honest ethically, culturally, economically, ecologically and scientifically, fully cognizant of place and community at whatever scales are of greatest significance, and integrative of both problem-response and opportunity, we may envision a mineral extraction and beneficiation future that achieves the best, most nearly sustainable, results possible. Industrial ecology (IE), with its clear emphasis on community, ecosystems, and sustainable economic stimulation, presents a sufficiently broad and adaptable discipline to facilitate planning and management toward sustainable mining. The creation of sustainable jobs encapsulates IE’s analogy to the niche survival strategies of organisms in nature. Open to common-sense recognition of faults and opportunities at appropriate scales, IE is less a discipline or prescribed methodology than a mindset, willing to be rigorously scientific when science is imperative, but also willing to exercise “art” when creativity or community engagement are needed. IE is also capable of future-looking compassion for peoples and nature. Looking beyond what has occurred to date, the case of Kennecott Utah Copper provides ample illustration of possibilities for community economic development, environmental management, and significant initiatives beneficial at regional and planetary scales, to become recognizable as deserving of the label, ‘sustainable.’ Through what this researcher terms “whole mine, whole region, whole planet” thinking, strategies become visible and subject to serious consideration, presenting a path to best possible accomplishment in mining. Constituting a maximal broadening of mining’s “traditional” scope, the integrative approach proposed here is the ‘paradigm shift’ being made by many other industries around the world. In aggregate, these steps are the only hope for avoidance of environmental, social and economic risks that threaten to curtail resource development and impair mining companies’ ‘social license to operate.’

Jan 1, 2005

By Vernon Helm, John S. Ball, Welton J. Wenger, William E. Haines

"INTRODUCTION Although the presence of sulfur causes many petroleum’s to be classified as ""low-grade"" because of the difficulties encountered in manufacturing products to meet rigid specifications, the petroleum industry is finding it necessary to use such crude oils to satisfy present needs. As the demand for petroleum products increases, and available reserves diminish, this utilization of high-sulfur crude oils will be accentuated. Programs designed to supplement the supplies of fuel available from petroleum by the manufacture of synthetic fuel from natural gas, shale oil, and coal are in progress. Such fuels, especially shale oil and, less so, oil from the hydrogenation of coal, also contain sulfur compounds which must be effectively removed. Thus, the need for fundamental research on the sulfur compounds that may be expected in petroleum and allied products is becoming greater as the industry is forced to call upon all available resources to meet the demand for petroleum products. In recognition of this need, the Bureau of Mines has inaugurated a long-range program assigned to furnish fundamental data concerning the presence, characteristics, and effects of sulfur compounds in petroleum and its products."

Dec 1, 1946

By Jr. Denton

This U.S. Bureau of Mines report presents the documentation for HEAPREC, a methodology for calculating reclamation performance bonds for cyanide heap leach operations. HEAPREC is a template developed for use with Lotus 1-2-33 release 2.01 or newer software. The report is presented in step-by-step "user's manual" format. Appendices contain detailed background and reference material on performance bonding, cyanide detoxification regulation, cyanide detoxification methods, general mine reclamation procedures, and an example bond calculation.

Jan 1, 1992

This report provides the results of an industrial engineering study that assessed the safety and efficiency of underground ore handling in U.S. metal mines. One of the primary findings of the study was the improvement needed in controlling muck size in the ore handling stream. Oversize ore causes much additional labor, creating work stoppages and is a principal cause of accidents in metal mines. This report includes data on block caving, back cut and fill room and pillar, shrinkage stoping, and sublevel stoping.

Jan 1, 1974

By James C. Barker

Alluvial cassiterite concentrations are widespread in river gravel and high level terraces within the Ray River watershed. The area lies in unglaciated terrain of Alaska's northern interior. Cassiterite originates from several calc-alkaline plutons of the Ruby batholith. Extensional stresses resulted in graben-like Tertiary basins that were flooded by a 200 ft thick section of mid-to late- Tertiary basalt flows. Basalt blocked local drainages, and 50- to 100-ft of terrace gravel was eventually deposited on top of the flows. Further downwarping and eventual fluvial downcutting of the flows resulted in cycles of accelerated sediment transport, deposition, and reconcentration. Repeated erosional cycles concentrated heavy minerals and resulted in development of tin placers. Preliminary resource estimates of contained tin in Recent alluvium range from 62 to as much as 172 million lbs-Sn in 300 million yd3. Grade of about 90% of the gravels is estimated to range between 0.2- to 0.5- lbs-Sn/yd3. Associated gold and rare earth elements (REE) in monazite and xenotime may be recoverable. Most of the tin is considered a subeconomic inferred resource, though, at least some of the gravels contain 2-to 6-lbs-sn/yd3. Estimates are projected on the basis of surface sampling and several auger drill holes and are provided for the purpose of land-use management planning.

Jan 1, 2011

By Wu Xueling

9K medium was used to isolate iron-oxidizing bacteria from 27 sump water samples collected from Dexing Cop-per Mine, Chengmen Mountain Mine and Yin Mountain Mine, Jiangxi Province, China. Lead acetate and silver nitrate were used to enrich metal-resistant iron-oxidizing bacteria from the above water samples. Isolate 16 was isolated from the sample taken at Dexing Copper Mine. Growth experiments indicated that when incubated under lead acetate (3,500 mg/L), isolate 16 still carried out ferrous ion oxidation, while isolate H1, a less resistant strain taken at Yin Mountain Mine, had a tolerance level of only 400 mg/L. When assayed with other metals, i.e., Ag+ (240 mg/L), Hg2+ (0.9 mg/L), Cu2+ (32,000 mg/L) and Mg2+ (22,500 mg/L), isolate 16 still carried out ferrous ion oxidation while, on the other hand, isolate H1 exhibited much lower tolerance levels, i.e., Ag+ (60 mg/L), Hg2+ (0.1 mg/L), Cu2+ (19,000 mg/L) and Mg2+ (13,500 mg/L). Based on 16S rDNA sequence data, isolate 16 and H1 were both identified as Acidithiobacillus ferrooxidans.

Jan 1, 2007